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can Agar plates degrade over time? why do old agar plates bubble when incubated?
I plated streptococcus onto old set of agar plates containing Ampicillan. nothing grew and was curious to know if agar plates can loose their nutrients? these were fairly old plates
Old agar plates will not necessarily "lose their nutrients", but they will dry out and crack up after a sufficient amount of time. Also, if the plates contain an antibiotic like Ampicillin, it is possible that the antibiotic will have degraded if the plates are old. Generally, I wouldn't use any antibiotic plates that are more than a couple of months old.
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